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“Sex” refers to the biological and physiological characteristics that define men and women.
“Gender” refers to the socially constructed roles, behaviours, activities, and attributes that a given society considers appropriate for men and women.
To put it another way: “Male” and “female” are sex categories, while “masculine” and “feminine” are gender categories.
Aspects of sex will not vary substantially between different human societies, while aspects of gender may vary greatly.